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KMID : 0368419990420020181
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Journal of Plant Biology
1999 Volume.42 No. 2 p.181 ~ p.185
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Functional Role of CAAT Box Element of the Nopaline Synthase(nos) Promoter
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Dai, Ziyu
An, Kyungsook/Edward, Gerald E./An, Gynheung
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Abstract
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The nopaline synthase (nos) promoter is active in a wide range of plant tissues and regulated by various environmental stimuli. It was previously found that the CAAT box region is important for nos promoter activity. In the present study, the location of the CAAT box element was determined by site specific mutation analysis. Point mutations within the conserved CAAT box element significantly reduced the promoter response in transgenic tobacco plants and calli to wounding, H_2O_2, methyl jasmonate, and 2,4-D, but not to salicylic acid. However, mutations immediately upstream from the CAAT box did not affect these responses. These results suggest that the CAAT box element is important in responding to certain stimuli.
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